Many Duluthians are unaware of it, but Aerostich motorcycle outfitters is based here. They manufacture high-quality protective motorcycle suits and jackets catering mostly to adventure/touring motorcyclists, and they sell them all over the world. It all happens from their building on 18th Ave West. If you see a motorcyclist wearing a debatably tacky-looking bright yellow motorcycle get-up, it's probably an Aerostich.

Does it have to be Duluth?  How about Steger Mulucks?  Or Windsong outterwear from Bayfield? Good quality products from both!

I would also say:

Field Logic hunting targets and products:  http://www.fieldlogic.com/

Genesis Attachments is a world-wide company:  http://www.genesisattachments.com

Michelinas (frozen foods):  http://www.michelinas.com

Although not very recognizable to someone outside of the industries I would say Altec Highline and Ikonics.

1. Lake Superior Brewery
2. Thirsty Pagan Brewery
3. Fitger's Brewhouse Beer
4. Maurice's
5. Decent human kindness

Jaeckel Organs, world-famous builder of pipe organs:
http://www.jaeckelorgans.com/index.html

Dorkus:

Whiskeyjack is now located in Montana. they departed Duluth about two years ago.

Is Ed Schaefer still making guitars locally?

Bucket Boss started in Duluth but was sold to Fiskars. Some of the original Bucket Boss folks started Duluth Trading Company, but I believe they actually operate out of Mt. Horab.

Diamond match company in Cloquet.

Jambox

If you really stretch the definition of the 'metro area' you could count Jack Link's Beef Jerky headquartered in Minong.

Sota Clothing is run by a UMD student. http://sotaclothing.com/

That Jeno's commercial was made by Sterling Cooper Agency.

Last I heard Ed Schafer moved to Texas. 

http://www.schaeferguitars.com/index.php

Lotus Guitar FX Pedals!!
http://www.lotuspedaldesigns.com/

Amsoil is a Superior based producer of synthetic motor oil:
http://www.amsoil.com/

They also have a nutritional supplement division, Altrum:
http://www.altrumonline.com/

Somewhere downtown there's evidence of the original Oreck's department store in the form of some terrazzo inlay on the floor of a entrance way .. I forget where.

Ikonics was brought up earlier... I know of a lot of people who think it's cool I live in the town Ikonics is in. Their products are really popular with glass sand carvers.

I should have said, SO...Ed moved back to Texas. He's from Hillsboro, which is a spit from where I grew up.